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107,216,218,220,222,223 Operative mortality may be greater in hypertrophic cardiomyopathy 1275 9 Structure-Based Kinetics by Time-Resolved X-ray Crystallography 205 Examples Advantages β-Lactamase  Farnesyltransferase  Myoglobin  PYP  L29WMyoglobin  p450cam  Isocitrate Deydrogenase  Disadvantages kinetic mechanism Trap-freeze Freeze-trap without temperature cycles Freeze-trap with temperature cycles Chemical stabilization mutations Analytical trapping PYP [21,22] Highest resolution Time scale Highest resolution Highest resolution Early and late Highest resolution Authentic intermediates preserved Separation of intermediates intermediates Separation of intermediates No cryo-artifacts No artifacts due to mutation forx Kinetic mechanism possible Time scale preserved can be determined Early and late intermedates Only slow processes Cryo-artifacts Time scale is lost Time-scale is lost Mixture of states Cryo-artifacts Only early interme- possible diates Time scale is lost Mixture of states Structural changes due ondicator mutation Resolution optilns signal-to-noise ratio lower possible Complex techniques Table 9.
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